GK021 ScienCell 人類T細(xì)胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit
ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。
北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。
GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit，產(chǎn)品簡介：
產(chǎn)品品牌; ScienCell
產(chǎn)品貨號：GK021
產(chǎn)品名稱：GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit
產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well
GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit， 產(chǎn)品說明：
ScienCell 的 GeneQuery™ 人 T 細(xì)胞受體信號通路 qPCR 陣列試劑盒 （GQH-TRS） 旨在促進(jìn)參與 T 細(xì)胞受體信號通路的 88 個關(guān)鍵基因的基因表達(dá)譜分析。如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例如下所示：
T細(xì)胞受體復(fù)合物成分：CD3D，CD3E，CD3G
輔助受體：CD4、CD8A、CD8B
輔助抑制劑：PDCD1、CTLA4
共刺激物：ICOS，CD28
激酶：FYN，ZAP70
MAPK途徑：KRAS，HRAS，NRAS，RAF1，MAP2K1，MAPK1，MAPK3
PI3K/AKT 通路：PI3K、AKT1、AKT2
IKK/NFKB途徑：NFKB，DAG1，BCL10，CARD11，MAP3K7，MALT1，CHUK，IKBKB，IKBKG
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。
ScienCell's GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:
· T cell receptor complex components: CD3D, CD3E, CD3G
· Co-receptors: CD4, CD8A, CD8B
· Co-inhibitors: PDCD1, CTLA4
· Co-stimulators: ICOS, CD28
· Kinases: FYN, ZAP70
· MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
· PI3K/AKT pathway: PI3Ks, AKT1, AKT2
· IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查詢™人 T 細(xì)胞受體信號通路 qPCR 陣列板布局*（8 個粗體和斜體控件）
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | AKT1 | CD274 | CD8A | DYRK1A | HRAS | MALT1 | NCK1 | NFKBIE | PDPK1 | PPP3CB | RELA | β-actin |
B | AKT2 | CD28 | CD8B | DYRK2 | ICOS | MAP2K1 | NCK2 | NRAS | PIK3CA | PPP3R1 | SOS1 | GAPDH |
C | BCL10 | CD3D | CDC42 | FOS | IKBKB | MAP2K7 | NFATC1 | NRON | PIK3CB | PRKCA | SOS2 | LDHA |
D | CALM1 | CD3E | CDK4 | FYN | IKBKG | MAP3K7 | NFATC2 | PAK1 | PIK3R1 | PRKCQ | TEC | NONO |
E | CARD11 | CD3G | CHUK | GRAP2 | ITK | MAP3K8 | NFATC3 | PAK2 | PIK3R2 | PTCRA | TNF | PPIH |
F | CBL | CD4 | CTLA4 | GRB2 | JUN | MAPK1 | NFKB1 | PAK3 | PIK3R3 | PTPN6 | VAV1 | GDC |
G | CBLB | CD80 | DAG1 | GSK3A | KRAS | MAPK3 | NFKBIA | PAK4 | PLCG1 | RAF1 | VAV2 | PPC |
H | CBLC | CD86 | DLG1 | GSK3B | LCP2 | MAPK9 | NFKBIB | PDCD1 | PPP3CA | RASGRP1 | ZAP70 | NTC |
提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新
ScienCell基因表達(dá)分析產(chǎn)品訂購信息：
GK001 人類細(xì)胞凋亡qPCR檢測試劑盒，Human Apoptosis qPCR Array Kit
GK002 人類多能干細(xì)胞qPCR檢測試劑盒，Human Pluripotent Stem Cell qPCR Array Kit
GK003 人類細(xì)胞周期qPCR檢測試劑盒，Human Cell Cycle qPCR Array Kit
GK005 人上皮細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Epithelial Cell Biology qPCR Array Kit
GK006 人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒，Human Neural Development and Regeneration qPCR Array Kit
GK007 人類神經(jīng)可塑性qPCR檢測試劑盒，Human Neural Plasticity qPCR Array Kit
GK008 人類神經(jīng)傳遞和膜轉(zhuǎn)運(yùn)qPCR檢測試劑盒，Human Neural Transmission and Membrane Trafficking qPCR Array Kit
GK009 人類星型膠質(zhì)細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Astrocyte Cell Biology qPCR Array Kit
GK010 人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測試劑盒，Human Melanocyte Development & Pigmentation qPCR Array Kit
GK011 人類黑素細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Melanocyte Cell Biology qPCR Array Kit
GK013 人類星型膠質(zhì)細(xì)胞轉(zhuǎn)錄因子qPCR檢測試劑盒，Human Astrocyte Transcription Factors qPCR Array Kit
GK017 人血管生成qPCR檢測試劑盒，Human Angiogenesis qPCR Array Kit
GK018 人內(nèi)皮細(xì)胞表型異質(zhì)性qPCR檢測試劑盒，Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit
GK019 人內(nèi)皮細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Endothelial Cell Biology qPCR Array Kit
GK020 人B細(xì)胞受體信號通路qPCR檢測試劑盒，Human B Cell Receptor Signaling Pathway qPCR Array Kit
GK021 人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit
GK022 人內(nèi)吞作用qPCR檢測試劑盒，Human Endocytosis qPCR Array Kit
